General Microbiology (FTAP2201)
BSc Food Science and Technology - FST
Semester: Second Semester
Level: 200
Year: 2019
THE UNIVERSITY OF BAMENDA COLLEGE OF TECHNOLOGY
2018 / 2019 Academic year Second Semester Exam
General Microbiology (FSAP 2202) Lecturer: Dr Njua Clarisse Yafi
Time allowed: 1hour 30mins
Section A: Multiple choice questions. Choose the letter corresponding to the most
correct/appropriate answer (15 marks). Spend no more than 30mins on this section.
1.
He provided the first evidence to disprove the theory of spontaneous generation.
a)
Louis Pasteur
b)
Francesco Redi
c)
Antony van Leeuwenhoek
d)
Robert Koch
2.
He finally settled the conflict over spontaneous generation with his famous swan-necked
flask experiments
a)
Louis Pasteur
b)
Francesco Redi
c)
Antony van Leeuwenhoek
d)
Robert Koch
3.
Who proposed the successive addition of the kingdoms Protista and Monera?
a)
Robert Whittaker
b)
Ernest Haeckel
c)
Carolus Linnaeus
d)
Carl Woese
4.
The binomial system of nomenclature was introduced by
a)
Robert Whittaker
b)
Ernest Haeckel
c)
Carolus Linnaeus
d)
Carl Woese
5.
Viruses are
a)
Unicellular
b)
Multicellular
c)
Acellular
d)
None of the above
6.
Virusoids
a)
Are short circular self-replicating DNA molecules
b)
Are short circular self-replicating RNA molecules
c)
Are non-self-replicating RNA molecules
d)
Are infectious proteins molecules
e)
None of the above
7.
The following are macroelements
a)
Carbon, nitrogen, sulfur, zinc
b)
Carbon, nitrogen, sulfur, iron
c)
Carbon, oxygen, nitrogen, copper
d)
Copper, cobalt, zinc, carbon
e)
Copper, cobalt, zinc, manganese
8.
The following are trace elements
a)
Carbon, nitrogen, sulfur, zinc
b)
Carbon, nitrogen, sulfur, iron
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c)
Carbon, oxygen, nitrogen, copper
d)
Copper, cobalt, zinc, carbon
e)
Copper, cobalt, zinc, manganese
9.
Heterotrophs
a)
Use organic compounds as their source of carbon
b)
Use inorganic compounds as their source of carbon
c)
Use CO
2
as their source of carbon
d)
Use both inorganic and organic compounds as carbon source
10.
Autotrophs
a)
Use organic compounds as their source of carbon
b)
Use inorganic compounds as their source of carbon
c)
Use CO
2
as their source of carbon
d)
Use both inorganic and organic compounds as carbon source
11.
Algae are
a)
Photolithotrophic autotrophs
b)
Photoorganotrophic heterotrophs
c)
Chemolithotrophic autotrophs
d)
Chemoorganotrophic heterotrophs
12 Green and purple non-sulfur bacteria are.
a)
Photolithotrophic autotrophs ,
5
b)
Photoorganotrophic heterotrophs
c)
Chemolithotrophic autotrophs
d)
Chemoorganotrophic heterotrophs
13. Most pathogenic bacteria are
a)
Photolithotrophic autotrophs
b)
Photoorganotrophic heterotrophs
c)
Chemolithotrophic autotrophs
d)
Chemoorganotrophic heterotrophs
14.
A culture medium that supports the growth of a wide range of microorganisms is called
a)
An enriched medium
b)
A general purpose medium
c)
A selective medium
d)
A differential medium
15.
A fastidious microorganism should be grown on
a)
An enriched medium
b)
A general purpose medium
c)
A selective medium
d)
A differential medium
16.
Obligate aerobes
a)
Can grow in the absence of oxygen
b)
Only grow in the presence of oxygen
c)
Can tolerate small amounts of oxygen
d)
Can grow in the presence or absence of oxygen
e)
Can't tolerate the presence of oxygen
17.
Facultative anaerobes
a)
Only grow in the presence of oxygen
b)
Can tolerate small amounts of oxygen
c)
Can grow in the presence or absence of oxygen
d)
Can't tolerate the presence of oxygen
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18.
Microaerophiles
a)
Can grow in the absence of oxygen
b)
Can tolerate small amounts of oxygen
c)
Can grow in the presence or absence of oxygen
d)
Can't tolerate the presence of oxygen
19.
Sterilization
a)
Completely destroys or removes all living cells, viable spores, viruses and viroids
b)
Kills, inhibits or removes all disease causing microorganisms from inanimate objects
c)
Considerably reduces the microbial population to acceptable public health standards
d)
Reduces pathogenic microorganisms on living tissues thus preventing infection
20.
Disinfection
a)
Completely destroys or removes all living cells, viable spores, viruses and viroids
b)
Kills, inhibits or removes all disease causing microorganisms from inanimate objects
c)
Considerably reduces the microbial population to acceptable public health standards
d)
Reduces pathogenic microorganisms on living tissues thus preventing infection
21.
Antisepsis
a)
Completely destroys or removes all living cells, viable spores, viruses and viroids
b)
Kills, inhibits or removes all disease causing microorganisms from inanimate objects
c)
Considerably reduces the microbial population to acceptable public health standards
d)
Reduces pathogenic microorganisms on living tissues thus preventing infection
22.
Sanitization
a)
Completely destroys or removes all living cells, viable spores, viruses and viroids
b)
Kills, inhibits or removes all disease causing microorganisms from inanimate objects
c)
Considerably reduces the microbial population to acceptable public health standards
d)
Reduces pathogenic microorganisms on living tissues thus preventing infection
23.
Fixation
a)
Doesn't kill the microorganisms
b)
Kills the microorganisms
c)
Attaches the specimen to the slide
d)
Preserves the integrity of cellular components
e)
All except "b" are correct
f)
All except "a" are correct
24.
Basic dyes are
a)
Positively charged
b)
Negatively charged
c)
Can be used as positive stains to stain bacterial cell walls
d)
Can be used as negative stains to stain the background
e)
"b" and "d" are not correct
25.
Acidic dyes
a)
Positively charged
b)
Negatively charged
c)
Can be used as positive stains to stain bacterial cell walls
d)
Can be used as negative stains to stain the background
e)
"b" and "d" are correct
26.
Simple staining techniques give information about
a)
The size and shape of cells
b)
The arrangement of cells
c)
Structural or compositional differences between microorganisms
d)
All the above are correct
e)
"c" is not correct
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27.
Differential staining techniques give information about
a)
The size and shape of cells
b)
The arrangement of cells
c)
Structural or compositional differences between microorganisms
d)
All the above are correct
e)
“c" is not correct
28. Parasites
a)
Are free-living
b)
Feed on death or decaying matter
c)
Can exist as normal flora or be pathogenic
d)
All the above are correct
9. An opportunistic pathogen
a)
Causes disease in individuals with intact immunological defenses
b)
Causes disease in immune-compromised individuals
c)
Can be part of the normal flora
d)
"a" and "c" are correct
e)
"b" and "c" are correct
30. Disease may result from a pathogen's
a)
Invasiveness
b)
Toxicity
c)
"a" and "b" are correct
d)
None is correct
Section B: Answer all questions and spend at least lhour on this section (20 marks)
1.
State: a) The germ theory of disease (1mk)
b) Koch's Postulates (2mks)
2.
Draw and label a typical prokaryotic cell (3mks)
3.
Name the two most common bacterial shapes giving all the possible ways in which they can
be arranged (3mks)
4.
Diagrammatically represent the growth curve of microorganisms growing in a closed system.
Clearly indicate all the phases of growth (3mks)
5.
a) Which groups of microorganisms can't tolerate the presence of oxygen and why? (2mks)
b) Which enzymes do some microorganisms possess that enables them to use oxygen or to
grow in its presence? (1mk)
6.
Give two differences between a light microscope and an electron microscope (1mk)
7.
a) Outline the main steps involved in the Gram Staining technique (2mks)
b) How do gram positive and gram negative bacteria appear when stained with Gram's stain
and why? (2mks)
Best wishes!!
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