Structural Biochemistry 1 (BCHS3101)

BSc, Biochemistry - BCHS

Semester: First Semester

Level: 300

Year: 2015

Answers (200 )
REPUBLIC OF CAMEROON
THE UNIVERSITY OF BAMENDA
Peace - work – Fatherland Box 39 Bambili, CAMEROON
School/Faculty: Science Department: Biochemistry Lecturers: Dr. Nantia & Mbouobda
Course Code: BCHS 33101 Course Title: Structural Biochemistry 1
Date: January 2016 Hall: Amphi & SOWETO Time: 08:00 – 10:00
Instructions: Answer questions in ordering manner and do not miss different exercise
Exercise 1: (4 marks) MCQ
1)
–
a
4)
–
c
6)
–
d
9)
–
c
2)
–
b
5)
–
d
7)
–
a
10)
–
a
3)
–
a
8)
–
a
11)
–
b
Exercise 2: (6 marks)
1) Determine the net charge and ionization form of the amino acids at the following pH (2 + 4 marks)
pH 3.05 pH 10.76
Amino acid Net Charge Ionization form Net Charge Ionization form
S
D
Q
R
Exercise 3: (10 marks)
A devoted student in the course of the isolation of protide has obtained the following:
NEW YEAR and HAPPY: Asn-Glu-Trp-Tyr-Glu-Ala-Arg His-Ala-Pro-Pro-Tyr
1) Justify the class(es) of these products (1 mark)
- Peptides: heptapeptide and pentapeptide
2) Compare their ionization behavior in increasing with addition steady quantity of strong base starting from highly
acidic medium (4 marks)
3) Determine their pI (1.5 marks)
4) Compare their mobility on electrophoretic current at pH 3 and 9 (1.5 marks)
5) Compare the action of buret reagents sulphur reagents on these protides (2 marks)
6) Positive reaction with buret and no reaction with suphur reagents
Given pKNH
2
: 9.6 pKCOOH: 1.9 pKgC: 8.3 pKgD: 3.9 pKgH: 6.0 pKgK:10.1 pKgR: 12.5
Exercise 4: (10 marks)
1) Give the structure and biological function of cAMP.UTP (4 marks)
It is second messenger: UTP also has the role of a source of energy or an activator of substances in metabolic reaction
2) In a table differentiate between the primary and secondary structure of DNA (4 marks)
Feature Primary Structure Primary Structure
Bond N-glycosidic, phosphodiester, H-Bond N-glycosidic, ester bonds
Component Nucleotide Mucleotide
Strand One Two
Configuration / Anti-parallel
3) 3) Match the type of bond with the role below (2 marks)
a- 4 b- 1 c- 2 d- 3
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