Structural Biochemistry 1 (XXXXX)
College of Technology (COLTECH)
Semester: First Semester
Level: 200
Year: 2014
T HE UNIVERSITY OF BAMENDA COLLEGE OF TECHNOLOGY
Department of Agriculture and Environmental Engineering
Structural Biochemistry I
Answer all the questions (2Hours30 minutes)
A-Nucleic acids (8.5 marks)
1- give the main functions of nucleic acids (2 marks)
2- give the main differences between DNA and RNA in terms of structure and function (3 marks)
3- Differences between hyperchromic and hypochromic effects (I 5 marks)
4- define the following: palindromic sequence, primer, plasmid, restriction enzyme (2 marks)
B-Lipids (8 marks)
1- Draw the structure of the followings a-phosphatidylglygerol, b-arachidic acid, c-oleic acid;
d-diacylglycerol (2 marks)
2- Define surfactants, and then give the equations of four of these surfactants
9
(2 5marks)
3- Define melting temperature then which fatty acid has the higher melting temperature and why?;
4- a) 18:1∆
9
b) 18:2 ∆
9 . 1 2
c) 18:0 d)18:l ∆" e) 18:3 ∆
9.12. l5
(l .5 marks).
5- Give four properties of lipids (2 marks)
C- Carbohydrates (9 marks)
1- An unknown substance containing only C, H, and 0 was isolated from goose liver A 0.423g sample
produced 0.620g of CO
2
and 0.254 g of H
2
O after complete combustion in excess oxygen Give the
equation of complete oxidation of this compound and the empirical formula considering that its being a
carbohydrate (3.5 marks)
2- Use periodate ion (IO
4
-
) to distinguish between pyranosides and furanosides forms? (1.5 marks)
3- Structural Analysis of a Polysaccharide A polysaccharide of unknown structure was isolated
subjected to exhaustive methylation, and hydrolyzed Analysis of the products revealed three methylated
sugars in the ratio 1:1:1 The sugars were 2,3,4-tri-O-methyi- glucopyranosyl, 2,3,4,5-O-methyl
-galactopyranosyl; and 1,3,4,5- O- methyl- fructofuranose. What is the structure of the polysaccharide?
(2.5 marks)
4- Draw the Haworth perspective formulas of the and forms of D –glucose, What feature
distinguishes the two forms? (2 marks).
D-Protcins (9.5marks)
1- A peptide sequence is the follow: ANGCHCMP.
a- Give the name of this peptide with three and all letters (2 marks)
b- What is the action of Trypsin, Carboxypeptidase A and (mercaptoethanol (I.5marks)
2- Draw the equation of total titration of Lysine, then determine the pI (pK NH
2
= 9.6; pK COOH =
2.18; pK. inside chain = 10.5) (2 marks)
3- Name and describe two methods to determine the NH
2
terminal groups (2 marks)
4- what are the characteristics of the a-helix in protein structure? (2 marks)
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