Structural Biochemistry 1 (BCHS3101)
BSc, Chemistry - CHMS
Semester: First Semester
Level: 300
Year: 2017
REPUBLIC OF CAMEROON
THE UNIVERSITY OF BAMENDA
Peace - work – Fatherland Box 39 Bambili, CAMEROON
School/Faculty: Science Department: Biochemistry Lecturers: Dr. Nantia & Mbouobda
Course Code: BCHS 33101 Course Title: Structural Biochemistry 1
Date: 18-04-2017 Hall: Asanji Time: 11:00 – 14:00
Instructions: Answer questions in ordering manner and do not miss different exercise
Exercise 1: (44 marks)
A) Using a peptide NAD fill the blank space in the table below: (12 marks)
pH
1
7
11
Structure at
Net charge
∆
pH
Electrophoretic mobility
Given: pKCOOH2.2,pK
R.D
3.9,pKNH
2
NH
2
9.2
B) After purification of peptide from a given source, a level 2 science student then endeavours to sequence it. To this end,
he used the following steps:
Deduce the polypeptide sequence that generated the following results:
a) The action of trypsin digestion releases the peptides: KMS, FYR, CAR
b) Chymotrypsin action yields RKMS and CAREY
c) After the action of dansyl chloride N-dansyl-C is obtained
d) Treatment of the peptide with anhydrous hydrazine at 100
o
C gives amino acid hydrazides and S
1) Give the name. 3 letter nomenclature and the developed structure of the peptide in a (8 marks)
2) Give the principle of each treatment (5 marks)
3) Illustrate the structure of N-dansyl – C (3 marks)
4) Determine with justification the structure of the peptide student actually purified (5 marks)
5) Give with justification the class of such peptide (1 mark)
6) What could be the expected result of the exposure of the peptide to the following agents? Justify your answer )10
marks)
i) UV radiations ii) 10% CuSO4 iii) Boiling at 100
O
C iv) Saturated (NH
4
)
2
SO
4
v) Isothiocyanate vi) Perfomic acid vii) Flurodinitrobenzene viii) Concentrated HNO
3
ix) Strong Alkaline pH x) Cyanogen bromide
Exercise 2: (26 marks)
A nucleotide has a sequence: pApUpGpCpC
1) Justify the type of nucleic acid it is? (1 +1 marks)
2) Give the developed structure (5 marks)
3) Give the action of the following enzymes
a- Ribonuclease 1 (1.5 marks)
b- Ribonuclease U2 (1.5 marks)
c- Snake venon phosphodiesterase (1.5 marks)
d- Spleen phoosphodiesterase (1.5 marks)
4) Define the following Restriction enzymes, Palindromic sequences, sticky ends, Blunt ends (4 marks)
5) Give the importance of restriction enzymes in genetic engineering (3 marks)
6) rRNA molecules are responsible for the formation of ribosomes which are the site of protein synthesis. Name the
different types of prokaryotic rRNA and give their main functions (1.5 + 4.5 marks)
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